Ralstonia eutropha TF93 is blocked in tat-mediated protein export.

Influence of Matric Potential on Survival and Activity of Genetically Engineered Ralstonia eutropha H850Lr

Although the application of biodegradative genetically engineered micro organisms (GEMs) for bioremediation is very promising, the risks of their release should be assessed before their introduction into the environment. Lux-marked Ralstonia eutropha H850Lr (formerly Alcaligenes eutrophus H850Lr) was introduced into sterile and non-sterile soil microcosms at matric potentials ?2.11, ?30, ?750, ...

The hydrogen-sensing apparatus in Ralstonia eutropha.

Molecular hydrogen is widely used by microorganisms as a source of energy. One of the best studied aerobic hydrogen oxidizers, the beta-proteobacterium Ralstonia eutropha (formerly Alcaligenes eutrophus), harbors two distinct [NiFe]-hydrogenases which catalyze the heterolytic cleavage of H2 into 2H+ and 2e-. The genes encoding the hydrogenase subunits are arranged in two large operons together ...

Phosphoenolpyruvate is a signal metabolite in transcriptional control of the cbb CO2 fixation operons in Ralstonia eutropha.

The two highly homologous cbb operons of the facultative chemoautotroph Ralstonia eutropha H16 encode most enzymes of the Calvin-Benson-Bassham carbon reduction cycle. Their transcriptional regulation was investigated both in vitro and in vivo to identify a metabolic signal involved in this process. For this purpose an in vitro transcription system employing the DNA-dependent RNA polymerase pur...

Application of the Plackett-Burman Statistical Design to Optimize Poly(β-hydroxybutyrate) Production by Ralstonia eutropha in Batch Culture

High level recombinant protein expression in Ralstonia eutropha using T7 RNA polymerase based amplification.

We report further development of a novel recombinant protein expression system based on the Gram-negative bacterium, Ralstonia eutropha. In this study, we were able to express soluble, active, organophosphohydrolase (OPH), a protein that is prone to inclusion body formation in Escherichia coli, at titers greater than 10 g/L in high cell density fermentation. This represents a titer that is appr...